Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound
Patent
1997-10-03
1999-03-30
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing oxygen-containing organic compound
4351736, 435190, 4352523, 43525233, 4353201, 435471, 435822, 536 232, C12P 760, C12N 904, C12N 1500, C12N 100
Patent
active
058887865
ABSTRACT:
An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid (hereinafter 2KLGA) at high yields from D-sorbitol, which is prepared by transforming, with said expression vector, a microorganism capable of producing L-sorbose at high yields from D-sorbitol, which has no or low 2KLGA-decomposing activity or a host microorganism having, in addition to the above-mentioned properties, no or low L-idonic acid-producing activity; and a process for producing 2KLGA, which comprises culturing said transformant in a medium containing D-sorbitol. According to the present invention, 2KLGA useful for the production of L-ascorbic acid can be produced with ease and in larger amounts by a single operation of culture.
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Balbas et al. (1990) Methods in Enzymology, vol. 185, pp. 14-37.
Hayashi Hiromi
Ishii Yoshinori
Niwa Mineo
Saito Yoshimasa
Yoshida Masaru
Fujisawa Pharmaceutical Co. Ltd.
Slobodyansky Elizabeth
Wax Robert A.
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