Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-07-25
1999-09-21
Prats, Francisco
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 72, 435100, 53612313, 536124, C12P 1926, C12P 1912, C07H 304
Patent
active
059553200
ABSTRACT:
Di-N-acetyl-D-chitobiose, which is easily available as a chitin decomposate or a chemical reagent, is reacted with a microorganisms-derived deacetylase to produce 2-acetylamino-4-O-(2-amino-2-deoxy-.beta.-D-glucopyranosyl)-2-deoxy-D-gluc ose of formula (1). ##STR1## The method is quantitative and efficiently produces the compound (1). The compound (1) and its salts are usable as substrates for measuring enzymatic activities, and also as starting substances in the production of various food materials and in the field of glyco-chain engineering.
REFERENCES:
Tokuyasu et al, Biosci. Biotech. Biochem. 60(10):1598-1603 (1996).
John et al, Proc. Natl. Acad. Sci. 90:625-629.
K. Tokuyasu et al., Chitin and Chitosan Research, 3, No. 2, pp. 142-143 (1997).
Mitsutomi et al., "Action Pattern of Aeromonas hydrophila Chitinase on Partially N-Acetylated Chitosan", Agric. Biol. Chem. 54(4), pp. 871-877, (1990).
Tokuyasu et al., "Deacetylation of chitin oligosaccharides of dp 2-4 by chitin deacetylase from Colletotrichum lindemuthianum", Carbohydrate Research 303 (1997) pp. 353-358.
Tokuyasu et al., "Purification and Characterization of Extracellular Chitin Deacetylase from Colletrotrichum lindemuthianum", Biosci. Biotech. Biochem. 60 (10), pp. 1598-1603 (1996).
Hamamatsu Shioka
Hayashi Kiyoshi
Kameyama Mayumi
Mori Yutaka
Ono Hiroshi
Director of National Food Research Institute, Ministry of Agricu
Prats Francisco
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