Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Enzyme inactivation by chemical treatment
Patent
1997-09-12
1999-11-16
Witz, Jean C.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Enzyme inactivation by chemical treatment
435188, 435228, 530404, 530405, 514 2, C12N 999, C12N 996, C12N 980, A01N 3718, C07K 100
Patent
active
059856319
ABSTRACT:
A method for preventing the activation of inactive, recombinant Helicobacter pylori apourease is presented. The method comprises contacting the apourease with a compound that modifies a sulfhydryl or lysyl residue of the urease. The covalently modified apourease is prevented from molecular aggregation.
REFERENCES:
patent: 5538729 (1996-07-01), Czinn et al.
patent: 5783436 (1998-07-01), Hausinger
Nagata et al. "Potent inhibitory action of the gastric proton pump inhibitor lansoprazole againest urease activity of Helicobacter pylori: Unique action selective for H. pylore cells," Atnimicrob. Agents Chemotherapy (Mar. 1993) 37(4): 769-774.
Fishbein et al. "Urease catalysis and structure," J. Biol. Chem. (Nov. 1973) 218(22): 7870-77.
Riddles et al. "Jack bean urease: VI. Determination of thiol and disulfide content; Reversible inactivation of te enzyme by the blocking of the unique cysteine residue," Biochimica Biophys. Acta (1983) 743: 115-120.
Blakely et al., "Thiolate-Nickel(II) Charge-Transfer Peaks in the Spectrum of the .beta.-Mercaptoethanol-Urease Complex", Biochimica et Biophysica Acta, 744:219-229 (1983).
Buchner et al., "Renaturation, Purification and Characterization of Recombinant F.sub.ab -Fragments Produced in Escherichia Coli", Bio/Technology, 9:157-162 (1991).
Czinn et al., "Oral Immunization against Helicobacter pylori", Infect Immun., 59:2359-2363 (1991).
Fagain, "Understanding and increasing protein stability", Biochimica et Biophysica Acta, 1252:1-14 (1995).
Kuhler et al., "Structure-Activity Relationship of Omeprazole and Analogues as Helicobacter pylori Urease Inhibitors", J. Med. Chem., 38:4906-4916 (1995).
Lee et al., "Oral Immunization with Recombinant Helicobacter pylori Urease Induces Secretory IgA Antibodies and Protects Mice from Challenge with Helicobacter felis", J. Inf. Dis., 172:161-172 (1995).
Martin et al., "Site-directed Mutagenesis of the Active Site Cysteine in Klebsiella aerogenes Urease", J. Biol. Chem., 267:20024-20027 (1992).
Mobley et al., "Microbial Ureases: Significance, Regulation, and Molecular Characterization", Microbiol. Rev., 53:85-108 (1989).
Mobley et al., "Molecular Biology of Microbial Ureases", Microbiol. Rev., 59:451-480 (1995).
Nagata et al., "Inhibitory Action of Lansoprazole and Its Analogs against Helicobacter pylori: Inhibition of Growth Is Not Related to Inhibition of Urease", Antimicrobial Agents and Chemotherapy, 39:567-570 (1995).
Nagata et al., "Growth Inhibition of Ureaplasma urealyticum by the Proton Pump Inhibitor Lansoprazole: Direct Attribution to Inhibition by Lansoprazole of Urease Activity and Urea-Induced ATP Synthesis in U. urealyticum", Antimicrobial Agents and Chemotherapy, 39:2187-2192 (1995).
Park et al., "Site-directed mutagenesis of Klebsiella aerogenes urease: Identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis", Protein Science, 2:1034-1041 (1993).
Park et al., "Requirement of Carbon Dioxide for in Vitro Assembly of the Urease Nickel Metallocenter", Science, 267:1156-1158 (1995).
Perez-Perez et al., Effects of Cations on Helicobacter pylori Urease Activity, Release, and Stability, Infection and Immunity, 62:299-302 (1994).
Sriwanthana et al., "Proteus mirabilis Urease: Histidine 320 of UreC Is Essential for Urea Hydrolysis and Nickel Ion Binding within the Native Enzyme", Infection and Immunity, 61:2570-2577 (1993).
Todd et al., "Purification and Characterization of the Nickel-containing Multicomponent Urease from Klebsiella aerogenes", J. Biol. Chem., 262:5963-5967 (1987).
Todd et al., "Reactivity of the Essential Thiol of Klebsiella aerogenes Urease", J. Biol. Chem., 266:10260-10267 (1991).
Todd et al., "Identification of the Essential Cysteine Residue in Klebsiella aerogenes Urease", J. Biol. Chem., 266:24327-24331 (1991).
Monath Thomas P.
Soman Gopalan
Thomas, Jr. William D.
Hanley Susan
OraVax-Merieux Co.
Witz Jean C.
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