Method for preventing superoxide damage to cells and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S069200, C435S071100, C435S071200

Reexamination Certificate

active

09955502

ABSTRACT:
A method of reducing superoxide damage to a cell is disclosed. In one embodiment, this method comprises the step of engineering the cell to produce more than a native amount of the YggX protein or its homolog, wherein the cells are rendered more resistant to superoxide damage.

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Pomposiello P.J. et al., J. Bact. 185:6624-6632, SoxRS-Regulated Expression and Genetic Analysis of the yggX Gene ofEscherichia coli; Nov. 2003.
Osborne, MJ et al., Protein Sci 2005 14:1673-1678; The solution structure of the oxidative stress-related protein YggX fromEscherichia coli.
J. Gralnick and D. Downs, “Protection from Superoxide Damage Associated with an Increased Level of the YggX Protein inSalmonella enterica,” PNAS 98(14):8030-8035, 2001.

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