Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-07-14
1999-12-21
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 724, 435 725, 435962, 436 63, 436 64, 436501, 436519, 436538, 436813, G01N 33574
Patent
active
060047623
ABSTRACT:
This invention provides a method for preserving cells which comprises the steps of (a) suspending cells in a physiologically-acceptable, isotonic medium; and (b) fixing the cells so suspended at a temperature of less than about 10.degree. C. under sufficiently hypertonic conditions so as to disperse the cells in a single, unagglutinated state, thereby preserving cells. This invention also provides a method for detecting cells separated from a sample which have been preserved according to the aforementioned method. This invention also provides a method for visualizing cells. Also provided is a method for detecting a metabolic process in cells present in a sample. This invention also provides a method for detecting the presence of rare cells in a sample which specifically possess on their surfaces a moiety recognized by a known ligand comprising preserving cells separated from the sample according to the aforementioned method for preserving cells. The subject invention also provides for quantitatively determining and isolating rare cells detected according to the aformentioned method. Further provided are methods for determining whether a fetus is afflicted with a genetic abnormality, determining the gender of a fetus, determining whether a subject is afflicted with a tumor, and detecting in a subject the presence of cells expressing a malignant phenotype, each method comprising preserving cells according to the aforementioned method for preserving cells.
REFERENCES:
patent: 4654312 (1987-03-01), Chang et al.
patent: 4680258 (1987-07-01), Hammerling et al.
patent: 4857300 (1989-08-01), Maksem
patent: 4902613 (1990-02-01), Chang et al.
patent: 5122453 (1992-06-01), Martin et al.
patent: 5134075 (1992-07-01), Hellstrom et al.
patent: 5192533 (1993-03-01), Elliott et al.
patent: 5192553 (1993-03-01), Boyse et al.
patent: 5236823 (1993-08-01), Adachi
patent: 5343741 (1994-09-01), Esposits et al.
patent: 5348741 (1994-09-01), Esposito et al.
patent: 5407805 (1995-04-01), Seon
patent: 5422277 (1995-06-01), Connelly et al.
patent: 5432054 (1995-07-01), Saunders et al.
patent: 5648222 (1997-07-01), Tse et al.
Ludgete, M. et al., "T-Cell Subset Analysis of Cryopreserved Human Peripheral Blood Mononuclear Cells," Immunology Letters, 7:119-123, 1983.
Paterson, D. et al., "Assessment of oestrogen receptor content of breast carcinoma by immunohistochemical techniques on fixed and frozen tissue and by biochemical ligand binding assay," Journal of Clinical Pathology, 43:46-51, 1990.
Takahashi, T. et al., "Osmotic Stress and the Freeze-Thow Cycle Cause Shedding of Fc and C3b Receptors by Human Polymorphonuclear Leukocytes," The Journal of Immunology, 134(6):4062-4068, Jun. 1985.
Van Dam, P. et al., "Comparative Evaluation of Fresh, Fixed, and Cryopreserved Solid Tumor Cells for Reliable Flow Cytometry of DNA and Tumor Associated Antigen," Cytometry, 13:722-729, 192
Zola, H., Monoclonal Antibodies: A Manual of Techniques, CRC Press, Inc., Boca Raton, Florida, pp. 112-115, 1987.
Bruch, J.F., et al. Trophoblast-Like Cells Sorted from Peripheral Maternal Blood Using Flow Cytometry: A Multiparametric Study Involving Transmission Electron Microscopy and Fetal DNA Amplification. Prenat. diagn. (1991) II:787-798.
Cacheux, V. et al. Detection of 47XYY Trophoblast Fetal Cells in Maternal Blood by Fluorescence in sity Hybridization after Using Immunomagnetic Lymphocyte Depletion and Flow Cytometry Sorting. Fetal Diagn. Ther (1992) 7:190-194.
Covone, A.E., et al. Analysis of Peripheral Maternal Blood Samples for the Presence of Placenta-Derived Cells Using Y-Specific Probes and McAb H315. Prenat. diagn. (1988) 8:591-607.
Psallidopoulos, M.C., et al. Integrated Proviral Human Immunodeficiency Virus Type 1 is Present in CD4+ Peripheral Blood Lymphocytes in Healthy Seropositive Individuals. Journal of Virology (1989) 63:4626-4631.
Tse, D.B. and Pernis, B. Spontaneous Internalization of Class I Major Histocompatibility Complex Molecules in T Lymphoid Cells. J. Exp. Med. Jan. (1984) 159:193-207.
Tse, D.B., et al. Intracellular Accumulation of T-Cell Receptor Complex Molecules in a Human T-Cell Line. Science (1986) 234:748-751.
Chung Hui-Min
Tse Doris B.
Van Der Ploeg Leonardus H. T.
Ceperley Mary E.
The Truatees of Columbia University in the City of New York
White John P.
LandOfFree
Method for preserving cells, and uses of said method does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for preserving cells, and uses of said method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preserving cells, and uses of said method will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-503669