Method for preservation of nitrile hydration activity

Details Method for preservation of nitrile hydration activity Method for preservation of nitrile hydration activity

1987-05-01

1990-02-13

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435227, C12N 996

Patent

active

049006720

ABSTRACT:
Nitrile hydration activity of nitrile hydratase produced by a microorganism of the genus Pseudomonas or Pseudomonas microorganism cells per se or in the immobilized state, all having nitrile hydration activity can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a suspension or solution of the nitrile hydratase, or the microorganism cells per se or in the immobilized state.

REFERENCES:
patent: 4390631 (1983-06-01), Watanabe et al.
patent: 4629700 (1986-12-01), Prevatt et al.
Bui et al., "A Note on the Enzymic Action and Biosynthesis of a Nitrile-Hydratase from a Brevibacterium Sp.", J. Appl. Bacteriol. 1984, 57(1), 183-190.
Fradet et al., "Hydratation of Nitriles Using a Bacterial Nitrile-Hydratase Immobilized on DEAE-Cellulose," Biotech. and Bioeng., vol. 27, No. 11, pp. 1581-1585 (1985).
Nagasawa et al., "Nitrile Hydratase of Pseudomonas Chlororaphis B23," Eur. J. Biochem. 162, 691-698 (Feb. 1987).

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