Method for preservation of nitrile hydration activity

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...

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435227, 435832, 435840, 435843, 435859, 435872, C12N 996

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active

049313916

ABSTRACT:
Nitrile hydration activity of the nitrilase or the immobilized from thereof can be stably preserved by adding as a stabilizer at least one compound selected from nitriles, amides, and organic acids and salts thereof to a solution or suspension of the nitrilase or of the immobilized form thereof.

REFERENCES:
patent: 4390631 (1983-06-01), Watanabe et al.
patent: 4629700 (1986-12-01), Prevatt et al.
Bui et al., "A Note on the Enzymic Action and Biosynthesis of a Nitrile-Hydratase from a Brevibacterium sp.," J. Appl. Bacterial. 1984, 57(1), 183-190, (CA 101:166185t).
Fradet et al., "Hydratation of Nitriles Using a Bacterial Nitrile-Hydratase Immobilized on DEAE-Cellulose," Biotechnol. and Bioeng., vol. 27, No. 11, pp. 1581-1585, (1985).
Nagasawa et al., "Nitrile Hydratase of Pseudomonas Chlororaphis B23," Eur. J. Biochem., 162, 619-698, (Feb. 1987).

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