Method for preservation and storage of viable biological materia

Refrigeration – Processes – Deodorizing – antisepticizing or providing special atmosphere

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62 64, 435 1, F24F 316

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active

046883878

ABSTRACT:
A method for preserving biological material is disclosed in which the biological material is stored at cryogenic temperatures for long periods of time without incurring fatal damage to cells, tissues or organs. The process comprises freezing the biological material under conditions of temperature and pressure to avoid the formation of crystalline ice I at all times during the freezing process. Rather, metastable phases of ice are exploited to reduce damage to the biological material upon freezing, storage or subsequent thawing.

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Mazur, Peter: Fundamental Cryobiology & Preservation of Organs by Freezing, Organ Preservation for Transplantation, Edit: A. M. Karow, Jr. & D. E. Pegg, Marcel Dekker, Inc., 2nd Edition, 1981, p. 144.
Fahey, G. M. et al.: "Prospects for Organ Preservation by Vitrification", Organ Preservation: Basic and Applied Aspects, Edit. D. E. Pegg, I. A. Jacobson, N. A. Halasz, MTP Press Limited, 1982, pp. 399-404.
Johnson, F. H. et al: "Kinetic Basis of Molecular Biology", John Wiley & Sons, Inc., 1954, pp. 286-368.

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