Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-01-27
1996-01-16
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 72, 435 75, 435 18, 435 712, 935 78, 935 81, C07H 2104, C12P 1934, C12Q 148, C12Q 170
Patent
active
054847028
ABSTRACT:
A method for preselecting a nucleic acid sequence of interest is provided. The method comprises immobilizing an affinity molecule to a solid support matrix, wherein the affinity molecule is specific for a hapten or target molecule; hybridization of a "hook" comprised of either an oligonucleotide (complementary to the sequence of interest) labeled with a hapten or target molecule to the target recombinant vector containing the nucleic acid sequence of interest; capturing of the vector-hook via the hapten or target molecule by the respective immobilized affinity molecule for which it has binding specificity; liberating the captured vector DNA by enzymatic digestion of either the hook, or of the immobilized affinity molecule; and adding and incubating competent host cells to promote the introduction of recombinant vector DNA into the competent host cells. Also disclosed are embodiments in which a) the hook is incorporated as part of the vector by hybridization to a denatured vector, followed by second strand synthesis to include the hook as part of the newly synthesized strand; and b) the hook is a protein that binds a genetic element within or adjacent to the nucleic acid sequence of interest, and the immobilized affinity molecule has binding specificity for the protein.
REFERENCES:
patent: 4656127 (1987-04-01), Mundy
patent: 4777129 (1988-10-01), Dattagupta et al.
patent: 4851331 (1989-07-01), Vary et al.
patent: 4865967 (1989-09-01), Shiraishi et al.
patent: 5118605 (1992-06-01), Urdea
Rigas et al., "Rapid plasmid library screening using RecA-coated biotinyld probes", Proceedings of the National Academy of Sciences USA, vol. 83, 1986, pp. 9591-9595.
Atkinson, T. et al. Nuc. Acids Res. 16(13):6232, 1988.
Singh, H. et al., Biotechniques 7(3):252-261, 1989.
Zhang, X-k. et al. Nature 355:441-446, 1992.
Hultman, T. et al. J. Biotechnology 35:229-238, 1994 (Jun.).
Kijay, J. M. A. et al. Biotechniques 16(4):657-662, 1994.
Jones W. Gary
Research Foundation of the State University of New York at Buffa
Tran Paul B.
LandOfFree
Method for preselecting recombinant clones containing a specific does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for preselecting recombinant clones containing a specific, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preselecting recombinant clones containing a specific will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-308791