Method for preparing xylitol with high yield using recycling...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

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C435S254220, C435S255400

Reexamination Certificate

active

07846702

ABSTRACT:
Provided is a process for continuously producing xylitol in high yield and productivity using a vacuum microfiltration bioreactor containing a fermentation medium for a strain of the genusCandida, which includes: 5 300 g/l of xylose, 1 10 g/l of urea, 1 10 g/l of potassium diphosphate, 0.01 1 g/l of magnesium sulfate, 0.1-10 mg/l of MnSO404H2O, 0.1 10 mg/l of CoCl2-6H2O, 0.1 10 mg/l of NaMoO4-2H2O, 0.1 10 mg/l of ZnSO4-7H2O, 0.1 10 mg/l of AlCl3-6H2O, 0.1 10 mg/l of CuCl2-2H2O, 0.01-5 mg/l of H3BO3, 1-100 mg/l of FeSO47H2O, 0.1-10 mg/l of ascorbic acid, 1-100 mg/l of biotin, 1-100 mg of choline, 1-200 mg/l of folic acid, 1-100 mg/l of inositol, 1-100 mg/l of nicotinic acid, 0.1-10 mg/l of p-aminobezoic acid, 1-100 mg/l of pantothernic acid, 0.1-10 mg/l of pyridoxine, 10-1,000 mg/l of riboflavin, and 1-100 mg/l of thiamine.

REFERENCES:
patent: 2004/0014185 (2004-01-01), Ojamo et al.
patent: 12-093188 (2000-04-01), None
patent: 99-65347 (1999-08-01), None
patent: 99-86862 (1999-12-01), None
patent: 00-76625 (2000-12-01), None
Choi et al “Production of xylitol in recycle fermentations ofCandida tropicalis” Biotechnology Letters 2000 V 22, pp. 1625-1628.
Kim et al., “Increased Xylitol Production Rate During Long-term Cell Recycle Fermentation ofCandida tropicalis,” Biotechnol Lett, vol. 26, No. 8, pp. 623-627 (2004).
Baishan et al., “Using Genetic Algorithms Coupling Neural Networks in a study of Xylitol Production: Medium Optimisation”, J. Process Biochemistry, 38:979-985 (2003).

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