Method for preparing tryptophan rich peptides

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

Reexamination Certificate

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C426S041000, C426S583000, C514S001100, C514S005600

Reexamination Certificate

active

07977066

ABSTRACT:
Described is a method for selectively isolating tryptophan containing peptides from an aqueous peptide mixture, including the steps of controlling the pH of the aqueous peptide mixture to 4.0-6.0, forming a precipitate of tryptophan containing peptides, and isolating the precipitated peptides.The aqueous peptide mixture is preferably obtained by enzymatic cleavage at acidic pH of a protein source. Further, peptides having a Trp content of 8-15 w/w % and the use thereof as a food additive and as an active ingredient in a medicament are described.

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patent: 2000-001500 (2000-01-01), None
ExPaSy Peptide Cutter. Pepsin Digestion on Alpha-lactalbumin (NP 776803), pp. 1-2, (2007).
NCBI Sequence Viewer, V. 2.0., NP 776803: Alpha-lactalbumin, pp. 1-3, (2007).
English Translation of DE 4130284 A1, 2008, pp. 1-2.
Markus, et al., “The Bovine Protein a-lactalbumin Increases the Plasma Ration of Tryptophan to the Other Large Neutral Amino Acids, and in Vulnerable Subjects Raises Brain Serotonin Activity, Reduces Cortisol Concentration, and Improves Mood Under Stress,”The American Journal of Clinical Nutrition, vol. 71, No. 6, 2000, pp. 1536-1544.

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