Method for preparing transformed plant

Details Method for preparing transformed plant Method for preparing transformed plant

1991-10-30

1993-12-21

Fox, David T.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

800205, 800DIG55, 43524049, 4352405, C12N 1512, C12N 500, A01H 104

Patent

active

052720721

ABSTRACT:
A transformed plant of Gramineae is prepared by culturing an anther of Gramineae in a callus induction medium and, at a stage immediately before the microspore begins to cause division or during the initial division, transducing a genetic substance into the microspore cell through a pore formed by a laser pulse thereby to express genetic information of the genetic substance.
According to the present invention, it is unnecessary to prepare protoplast and therefore, time and operations for transformation can be greatly reduced. Since haploid cells are transformed, the character transduced is conveyed without separating at a later generation. In addition, difficulties in experiments between species and strains are minimized so that it is easy to apply the present invention to practical species. According to the present invention, large pores can be formed as compared to the electroporation method so that DNA or substances having a large molecular weight can be introduced.

REFERENCES:
patent: 4840906 (1989-06-01), Hunter et al.
Finn Lok Olsen, "Induction of Microspore Embryogenesis in Cultured Anthers of Hordeum Vulgare. The Effects of Ammonium Nitrate, Glutamine and Asparagine as Nitrogen Sources", Carlsberg Res. Commun., vol. 52, pp. 393-404 (1987).
K. J. Kasha et al., "Haploids in Cereal Improvement: Anther and Microspore Culture", Gene Manipulation in Plant Improvement II, Plenum Press, New York, pp. 213-235 (1990).
D. Clapham et al., "Haploid Hordeum Plants from Anthers in Vitro", Z. Pflanzenzuchtg, 69, pp. 142-155 (1973).
Michael W. Lassner et al., "Simultaneous Amplification of Multiple DNA Fragments by Polymerase Chain Reaction in the Analysis of Transgenic Plants and Their Progeny", Plant Molecular Biology Reporter, vol. 7(2), pp. 116-128 (1989).
A. Ziauddin et al., "Improved Plant Regeneration from Shed Microspore Culture in Barley (Hordeum vulgare L.) CV. Igri", Plant Cell Reports, vol. 9, pp. 69-72 (1990).
B. Foroughi-Wehr et al., "Plant Production from Cultured Anthers of Hordeum vulgare L.", Z. Pflanzenzuchtg., vol. 77, pp. 198-204 (1976).
Randall K. Saiki et al., "Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase", Science, vol. 239, pp. 487-491 (Jan. 29, 1988).
Ingo Potrykus, "Gene Transfer to Cereals: An Assessment", Biotechnology, pp. 535-542 (Jun. 1990).
Weber, G., et al., Plant Cell, Tissue, and Organ Culture, vol. 12, (1988), pp. 219-222.

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