Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1991-01-28
1993-03-30
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
25217412, 25217413, 25217414, C12N 996, C11D 1700
Patent
active
051983532
DESCRIPTION:
BRIEF SUMMARY
This application is a continuation of Ser. No PCT/DK89/00172, filed Jul. 11, 1989.
TECHNICAL FIELD
The present invention relates to stabilized enzyme dispersions.
BACKGROUND ART
Ensuring sufficient enzyme stability during storage represents a problem in the formulation of liquid enzymatic systems such as liquid enzymatic detergents, particularly those containing a detergent builder The problem has received considerable attention in the prior art. One approach has been incorporation of various chemicals as enzyme stabilizers.
Another approach has been to coat or encapsulate the enzyme with a suitable coating agent and disperse the coated enzyme in the liquid detergent.
Thus, the method described in EP-A-0238216 entails dispersing enzymes as particles in liquid detergent which has a structure which prevents sedimentation of the particles, after coating the particles with a hydrophobic, water-insoluble substance such as a silicone which isolates the particles from the aggressive medium. U.S. Pat. No. 4,090,973 describes encapsulating the enzyme in a water-soluble, solid surface active agent, such as polyvinyl alcohol or polyethylene glycol before addition to the liquid detergent. JP-A 63-105,098 describes coating of enzymes with polyvinyl alcohol to form microcapsules and dispersing the capsules uniformly in a liquid detergent to improve storage stability.
The methods according to said publications involve physically surrounding a particle or droplet containing the enzyme with a barrier which isolates the enzyme more or less effectively from the detergent medium. To ensure effective coating or encapsulation of the enzyme with a protective material, a relatively high amount of the latter is required.
One method, described in EP-A 0,238,216, is to protect the enzyme by dispersing it in a hydrophobic liquid which is insoluble in the detergent, such as silicone oil, and dispersing the liquid in the detergent. Another proposed method is to encapsulate the enzyme in non-ionic surfactant (U.S. Pat. No. 4,090,973) or polyvinyl alcohol (GB 1,204,123, JP-A 63-105,098, FR 2,132,216) by physically coating solid particles of enzyme with the encapsulant. JP-A 61-254,244 describes dispersing an enzyme in an aqueous polymer solution, dispersing the latter in a hydrocarbon and precipitating the polymer to form the micro capsules
SUMMARY OF THE INVENTION
We have found that when water soluble polymers are precipitated from aqueous solution to form a dispersion in the water and either the precipitation is effected in the presence of dissolved or finely dispersed enzyme, or the precipitate is subsequently contacted with dissolved or finely dispersed enzyme, so as to form a codispersion in water of the enzyme and polymer, substantial improvement of the enzyme stability during storage can be obtained with surprisingly little polymer (relative to enzyme). Our observation that enzyme stabilization can, surprisingly, even be obtained by contacting precipitated polymer with dissolved enzyme, leads us to believe that the stabilizing effect is not due (or at least not primarily due) to encapsulation.
Our invention, therefore, provides a method for the preparation of a stabilized aqueous enzyme dispersion comprising:
(1) precipitating a water-soluble polymer from aqueous solution to form an aqueous dispersion, and
(2) before, after or simultaneously with (1), contacting the dissolved or dispersed polymer with an aqueous solution or fine aqueous dispersion of enzyme.
A particularly preferred method comprises coprecipitation of enzyme and polymer from a solution comprising both of these or precipitation of the polymer in the presence of the dissolved enzyme. The stabilized enzyme dispersion according to the invention may in particular be an enzymatic liquid detergent or an enzymatic detergent additive.
DETAILED DESCRIPTION OF THE INVENTION
Enzyme
Typically the enzyme used in the invention is a protease, lipase, cellulase, amylase or other stain and/or soil removing enzyme. Mixtures of enzymes may be employed. For use in a liq
REFERENCES:
patent: 3557002 (1971-01-01), McCarty
patent: 3627688 (1971-12-01), McCarty et al.
patent: 3629123 (1971-12-01), O'Reilly et al.
patent: 3634258 (1972-01-01), Wildi et al.
patent: 3714051 (1973-01-01), Milesi et al.
patent: 3723250 (1973-03-01), Aunstrup et al.
patent: 3860484 (1975-01-01), O'Malley
patent: 3860536 (1975-01-01), Landwerden et al.
patent: 4090973 (1978-05-01), Maguire, Jr. et al.
patent: 4203857 (1980-05-01), Dugan
patent: 4250255 (1981-02-01), Sanford
patent: 4526698 (1985-07-01), Kuroda et al.
patent: 4707287 (1987-11-01), Herdeman
patent: 4743394 (1988-05-01), Kaufman et al.
patent: 4767557 (1988-08-01), Herdeman
Chadwick Philip
Hawkins John
Lykke Mads
Messenger Edward T.
Albright & Wilson Limited
Lambiris Elias J.
Lilling Herbert J.
Meller Mike
Novo Nordisk A S
LandOfFree
Method for preparing stabilized enzyme dispersion does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for preparing stabilized enzyme dispersion, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing stabilized enzyme dispersion will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1280555