Method for preparing soluble and active recombinant proteins...

Details Method for preparing soluble and active recombinant proteins... Method for preparing soluble and active recombinant proteins...

2005-10-24

2011-12-20

Desai, Anand (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S070100, C536S023100

Reexamination Certificate

active

08080387

ABSTRACT:
The present invention relates to a method for producing a recombinant protein capable of increasing expression rate of a target protein and also improving solubility and folding of the expressed target protein using a modified protein disulfide isomerase (PDI) as a fusion partner, and an expression vector containing the modified PDI gene as a fusion partner. The method for preparing a recombinant protein using a modified PDI as a fusion partner according to the present invention may solve the problems concerning a low yield and solubility and folding that conventional fusion partners have, and be widely used for protein drug and industrial protein production.

REFERENCES:
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patent: 2004/0018596 (2004-01-01), Udaka et al.
patent: WO 2007049829 (2007-05-01), None
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