Method for preparing sample for use in endotoxin test

Chemistry: analytical and immunological testing – For preexisting immune complex or auto-immune disease

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210635, 210927, 436178, 436825, G01N 3348, G01N 3354

Patent

active

044143362

ABSTRACT:
(1) A method for preparing a sample for use in the endotoxin test which comprises subjecting a sample of plasma or blood preparation to a contact with water-insoluble anti-human .alpha..sub.2 -macroglobulin antibody and to a gel filtration by means of a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, and recovering the maximum molecular weight fraction.
(2) A kit for preparing a sample for use in the endotoxin test consisting of a set of a sample-developing column prepared by packing a water-insoluble anti-human .alpha..sub.2 -macroglobulin antibody and a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution of a high molecular colored substance to be added to the sample and a buffer solution for use in developing the sample.

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