Method for preparing sample for use in endotoxin test

Details Method for preparing sample for use in endotoxin test Method for preparing sample for use in endotoxin test

1981-09-29

1983-11-08

Turk, Arnold

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

210635, 210927, 436178, 436825, G01N 3348, G01N 3354

Patent

active

044143362

ABSTRACT:
(1) A method for preparing a sample for use in the endotoxin test which comprises subjecting a sample of plasma or blood preparation to a contact with water-insoluble anti-human .alpha..sub.2 -macroglobulin antibody and to a gel filtration by means of a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, and recovering the maximum molecular weight fraction.
(2) A kit for preparing a sample for use in the endotoxin test consisting of a set of a sample-developing column prepared by packing a water-insoluble anti-human .alpha..sub.2 -macroglobulin antibody and a gel of allyl dextran crosslinked with N,N'-methylenebis acrylamide or a gel of polyvinyl alcohol having many hydrophilic hydroxyl groups, both equilibrated with a buffer solution of pH 7.0-7.4, into a column and tightly sealing the column, an aqueous solution of a high molecular colored substance to be added to the sample and a buffer solution for use in developing the sample.

REFERENCES:
patent: 3944391 (1976-03-01), Harris et al.
patent: 4107077 (1978-08-01), Sullivan et al.
patent: 4138474 (1979-02-01), Updike
patent: 4273557 (1981-06-01), Juranas
patent: 4276050 (1981-06-01), Firca et al.
patent: 4279774 (1981-07-01), Lindsay et al.
patent: 4350760 (1982-09-01), Nicolas et al.
Schenkein et al., "The Journal of Clinical Investigation", vol. 50, 1971, pp. 1864-1868.

Affiliated with

Also associated with

Rating

Method for preparing sample for use in endotoxin test does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for preparing sample for use in endotoxin test, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing sample for use in endotoxin test will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2008715