Method for preparing pertussis toxin toxoid using HcHo and amino

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...

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424 88, 424 92, 435193, 435194, 530405, 530406, 530410, 530415, 530416, 530825, A61K 3910, C12N 912, C07K 308, C07K 1504

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049962990

ABSTRACT:
Disclosed is a method for preparing pertussis toxin toxoid by a treatment pertussis toxin with formaldehyde, which comprises carrying out said treatment in the presence of lysine or glycine in combination with N-acetyltriptophan. The resultant toxoid does not exhibit toxicity reversion, thereby making the toxoid highly suitable for use in pertussis vaccines.

REFERENCES:
patent: 4762710 (1988-08-01), Sekura
patent: 4784589 (1988-11-01), Robinson et al.
patent: 4788058 (1988-11-01), Parton et al.
patent: 4845036 (1989-07-01), Burns et al.
patent: 4849358 (1989-07-01), Chazono et al.
patent: 4885359 (1989-12-01), Ginnaga et al.
Journal of Bacteriology in Japan, 42, 288, (1987), Sato et al.

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