Method for preparing peptides and proteins utilizing ribosome

Details Method for preparing peptides and proteins utilizing ribosome Method for preparing peptides and proteins utilizing ribosome

1994-06-28

1996-09-17

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 681, 935 44, 935 45, C12P 2102

Patent

active

055567681

ABSTRACT:
A novel method for synthesizing peptides and proteins having a desired amino acid sequence by utilizing an aminoacyl-tRNA and misaminoacyl-tRNA as well as an artificial mRNA in a system for in vitro protein synthesis is provided. According to the present method, desired peptides and proteins can be synthesized using naturally-occurring and non naturally-occurring amino acids. Accordingly, the present invention provides a fundamental technique which is important in the fields of various industries and various fundamental researches such as biochemistry, molecular biology and protein engineering.

REFERENCES:
McKay, F. C., et al, J. Am. Chem. Soc., 79, 4686-4690 (1957).
Carpino, L. A., et al, J. Am. Chem. Soc., 92, 5748-5749 (1970).
Merrifield, R. B., J. Am. Chem. Soc., 85, 2149-2154 (1963).
Bergmann, M., et al, J. Biol. Chem., 124, 1-6 (1938).
Hedgpeth, J., et al, Proc. Nat. Acad. Sci. USA, 69, No. 11, 3448-3452 (1972).
Nirenberg, M. W., et al, Proc. Nat. Acad. Sci. USA, 47, 1588-1602 (1961).
Oka, T., et al, J. Biochem. 84, 1277-1283 (1978).
Takai, H., et al, Peptide Chemistry 1980, K. Okawa, ed., Protein Research Foundation, 213-218 (1981).
Nakajima, H., et al, Biochimica et Biophysica Acta 790, 197-199 (1984).
Spirin, A. S., et al, Science, 242, 1162-1164 (1988).
Heckler, T. G., et al, Tetrahedron, 40, 87-94 (1984).
Heckler, T. G., Biochemistry, 23, 1468-1473 (1984).
Heckler, T. G., et al, J. Biol., Chem., 258, 4492-4495 (1983).
Roesser, J. R., et al, Biochemistry, 25, 6361-6365 (1986).
Payne, R. C., et al, Biochemistry, 26, 3197-3205 (1987).
Noren, C. J., et al, Science, 244, 182-188 (1989).
Noll, M., et al, J. Mol. Biol., 75, 281-294 (1973).
Usman, N., et al, J. Amer. Chem. Soc., 109, 7845-7854 (1987).
Ogilvie, K. K., et al, Proc. Natl. Acad. Sci. USA, 5764-5768 (Aug. 1988).
Spedding, G., "Ribosomes and Protein Synthesis," G. Spedding, ed., Oxford University Press, pp. 22-27 (1989).

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