Method for preparing optically active amino acids and their este

Details Method for preparing optically active amino acids and their este Method for preparing optically active amino acids and their este

1995-05-26

1996-09-03

Naff, David M.

Chemistry: molecular biology and microbiology

Process of utilizing an enzyme or micro-organism to destroy...

Resolution of optical isomers or purification of organic...

G12P 4100

Patent

active

055523188

ABSTRACT:
A process for the preparation of optically active D-amino acid and its esters, comprising the steps of: (a) preparing a reaction mixture containing an DL-amino acid ester and a lipase or an acylase in an aqueous solution; (b) reacting the reaction mixture at temperatures between 4.degree. and 60.degree. C.; and (c) using an organic extractant to separate the reaction mixture into an upper layer, which contains an optically active D-amino acid ester, and a lower layer, which contains L-homophenylalanine. The optically active D-amino acid ester can be chemically hydrolyzed to obtain an optically active D-amino acid The lipase can be selected from the group consisting of Aspergillus niger lipase, Pseudomonas sp. lipase, Rhizopus sp. lipase, porvine pancreas lipase, wheat germ lipase, and hog pancreas lipase, and the acylase can be porcine kidney acylase. Many of these lipases or acylase provide an enantiometric excess of more than 95%, and the amino acid can be selected from the group consisting of homophenylalanine, methionine, lysine, arginine, phenylalanine, p-chlorophenylanine, tyrosine, dopa, tryptophan, histidine, threonine, and phenylglycine.

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