Method for preparing nucleic acids for analysis and kits useful

Details Method for preparing nucleic acids for analysis and kits useful Method for preparing nucleic acids for analysis and kits useful

1994-09-21

1996-07-23

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 911, 435270, 435183, 536 231, 536 7433, 536 753, 935 76, 935 77, 935 78, C17Q 168, C17P 134, C07H 2102, C17N 1500

Patent

active

055388701

ABSTRACT:
Methods useful in purifying nucleic acids from whole cell samples are described. This is accomplished by adding one or both of a non-phenyl group containing non-ionic detergent or a cross-linked, polycarboxylic acid. Kits which can be used in the methods are also described.

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Jones et al., "A Rapid Method for Purication of Target DNA from Complex Biological And Environment Samples For Polymerase Chain Reaction (PCR) DNA Amplification" Abtract p-10, 93rd General Meeting of the American Society For Microbiology, May 16-20.
Haselbeck et al., "Studies on the effect of the incubation conditions, various detergents and protein concentration on the enzymatic activity of N-glycosidase R (Glycopeptidase F), and Endoglycosidase F", Topics In Biochemistry 8: 1-4 (1988).
Ehrlich et al., PCR Technology: Principles and Applications for DNA Amplification, pp. 19-21.
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Gibson et al., "A Simple And Rapid Method For Detecting Human Immunodefiency Virus By PCR", J. Virol. Meth. 32: 286 (1991).
Blin et al., "A General Method For Isolation of High Molecular Weight DNA from Eukaryotes", Nucl. Acid Res. 3(9): 2303-2308 (1976).

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