Method for preparing monoclonal antibody to Hbsag

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide

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43524027, 935 90, 935100, C12P 2100, C12N 500

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active

048837520

ABSTRACT:
Disclosed is a monoclonal antibody to HBsAg which is prepared by forming hybridomas between human peripheral blood lymphocyte cells, derived from humans having high titers of anti-HBsAg, and myeloma cells, cloning the hybridomas and selecting the antibody-producing clones. The monoclonal antibody can react with all the subtypes of HBsAg and is expected to be very effective in diagnosis and treatment of diseases due to the viral infection.

REFERENCES:
patent: 4271145 (1981-06-01), Wands et al.
patent: 4689299 (1987-08-01), Insel et al.
Ichimori, Y. et al., Biochem, Biophys. Res. Comm. 129, (1), 26-33, 1985.
"Continuous cultures of fused cells secreting antibody of predefined speccity", by G. Kohler et al., Nature, Vol. 256, 1975, pp. 495-497.
"Fusion Between Immunoglobulin-Secreting and Nonsecreting Myeloma Cell Lines", by G. Kohler et al., Eur. J. Immunol., Vol. 6, 1976, pp. 292-295.

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