Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1995-02-09
1996-07-30
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
435179, 435175, 435176, 435177, 435180, 435181, 435201, 435202, 435204, 435205, 530810, C12N 996, C12N 926, C12N 1100, C12N 1114
Patent
active
055410979
ABSTRACT:
A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.
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Emneus, J., et al., "Comparison Between Different Inorganic Supports for the Immobilization of Amyloglucosidase and .alpha.-lamylase To be Used in Enzyme Reactors in Flow-Injection Systems", Analy. Chim. Acta, 276:303-318, 1993.
Brewer Jack W.
Lantero Oreste J.
Sarber Sharon M.
Degen Nancy J.
Fleisher Mindy
Solvay Enzymes, Inc.
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