Method for preparing immobilized enzyme conjugates and immobiliz

Details Method for preparing immobilized enzyme conjugates and immobiliz Method for preparing immobilized enzyme conjugates and immobiliz

1995-01-09

1995-12-05

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435179, 435175, 435176, 435177, 435180, 435181, 435201, 435202, 435204, 435205, 530810, C12N 996, C12N 926, C12N 1100, C12N 1114

Patent

active

054728614

ABSTRACT:
A method for preparing an immobilized enzyme conjugate, whereby the enzyme is treated with a polyfunctional amine reactive material for forming a treated enzyme-containing adduct before being immobilized on a solid support which has been contacted with a solution of a polyamine compound. The method is especially preferred for use with glucoamylase, fungal .alpha.-amylase and .beta.-amylase. Immobilized enzyme conjugates formed by use of this method include treated enzyme-containing adducts. The immobilized enzyme conjugates disclosed herein are more stable and the enzymes immobilized therein are more tightly-held than those otherwise obtained and provided.

REFERENCES:
patent: 4355105 (1982-10-01), Lantero, Jr.
patent: 4713333 (1987-12-01), Chiang et al.
Food Chemicals Codex, Third Edition (1981), pp. 484-485.
Zemek, J., "Crosslinked polyethylenimine: An Enzyme Carrier with Spacers of Various Lengths Introduced in Crosslinking Reaction", Enzyme Microb. Technol., 1982, vol. 4, Jul., pp. 233-235.
Emneus, J., et al., "Comparison Between Different Inorganic Supports for the Immobilization of Amyloglucosidase and .alpha.-lamylase to be Used in Enzyme Reactors in Flow-Injection Systems", Analy. Chim. Acta, 276:303-318, 1993.
Wingard, Lemuel, "Enzyme Engineering", Biotechnology and Bioengineering Symposium, No. 3, 1972.

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