Method for preparing high molecular weight meningococcal Group C

Details Method for preparing high molecular weight meningococcal Group C Method for preparing high molecular weight meningococcal Group C

1977-08-01

1978-10-31

Rosen, Sam

Drug, bio-affecting and body treating compositions

Whole live micro-organism, cell, or virus containing

Genetically modified micro-organism, cell, or virus

A61K 3902

Patent

active

041235207

ABSTRACT:
A vaccine against Group C meningococcal meningitis consisting of a polysaccharide of which at least 80% by weight has a molecular weight in excess of 1,000,000 daltons is prepared. The polysaccharide was isolated from Group C hexadecyl trimethylammonium bromide cell paste with 1.0M CaCl.sub.2 extraction and purified by phenol extraction, ultracentrifugation at 100,000 g. and ethanol fractionation (30%-45% v/v in ethanol).

REFERENCES:
bhattacharjee et al - Chem. Abst., vol. 84 (1976) p. 134, 003w.
Limjuco et al - Chem. Abst., vol. 85 (1976) p. 175 449k.
Apicella - Chem. Abst., vol. 85 (1976) p. 92 038z.
Zollinger et al - Chem. Abst., vol. 78 (1973) p. 143 65v.

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