Method for preparing from cultured cells a thromboplastic reagen

Details Method for preparing from cultured cells a thromboplastic reagen Method for preparing from cultured cells a thromboplastic reagen

1994-06-21

1995-05-09

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 701, 435 41, 435 13, C12P 2102, C12P 100, C12Q 156

Patent

active

054139198

ABSTRACT:
A method for preparing a thromboplastin reagent from cultured human cells comprising the steps of washing the cells with isotonic aqueous salt solution, lysing the cells by hypotonic shock, and resuspending the cell lysate in a diluent compatible with a one-stage prothrombin time determination to reach a concentration of about 0.5 to 3.0 mg/mL thromboplastin, whereby the cell lysate thromboplastin suspension produces a clot in a one stage prothrombin time test in about 10 to 15 seconds, with a mean normal prothrombin time of about 11-13 seconds, when added to citrated normal plasma in a volume ratio of about 2:1, such thromboplastin reagent being stable for at least 8 hours at 37.degree. C. and for at least 5 days at 4.degree. C.

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