Method for preparing conjugates of proteins and chelating...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Metal containing – e.g. – chromoproteins – ferritin,...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C514S006900

Reexamination Certificate

active

11000500

ABSTRACT:
Improved synthesis methods are provided for preparing conjugates of proteins and chelating agents. In the synthesis methods, non-stable bonds are hydrolyzed after the conjugate is synthesized, for example by treating the conjugate with a quenching agent. The synthesis method provides conjugates that are substantially free of non-stable bonds between the protein and the chelating agent, such that the chelating agent is less likely to dissociate with the conjugate after the conjugate has been stored for extended periods. The conjugate may be useful for therapeutic or diagnostic methods.

REFERENCES:
patent: 5106955 (1992-04-01), Endo et al.
patent: 2003/0124057 (2003-07-01), Griffiths et al.
Ruble, et al., Int. J. Radiation OntologyBiol. Phys., 1996, 34, 609-616.
Timkovich, Analytical Chemistry, 1977, 79, 135-143.
[Retrieved from] “http://machaut.uchicago.edu/cgi-bin/WEBSTER.sh?WORD=subsequently”2007, 1 page [Retrieved on Feb. 28, 2007].
Ruble, Gaye, et al., “The Use of 212Pb-Labeled Monoclonal Antibody in the Treatment of Murine Erythroleukemia” Int. J. Radiation Oncology Biol. Phys., vol. 34, No. 3, pp. 609-616, 1996.
Timkovich, Russell, “Detection of the Stable Addition of Carbodiimide to proteins,” Analytical Biochemistry 79, 135-143 (1977).
Brinkley, Michael, “A Brief Survey of Methods for Preparing Protein Conjugates with Dyes, Haptens, and Cross-Linking Reagents” Teaching Editorial Bioconjugate Chem., vol. 3, No. 1, 1992.
Lewis, Michael R., “A Facile, Water-Soluble Method for Modification of Proteins with DOTA. Use of Elecvated Temperature and Optimized pH to Achieve High Specific Activity and High Chelate Stability in Radiolabeled Immunoconjugates” Bioconjugate Chem. 1994, 5, 565-576.
Govindan, Serengulam V., “90Yttrium-Labeled Complementarity—Determining-Region-Grafted Monoclonal Antibodies for Radioimmunotherapy: Radiolabeling and Animal Biodistribution Studies” Bioconjugate Chemistry reprinted from vol. 9, No. 6, pp. 773-782.
Carraway, Kermit L., et al., “Short Communication: Reaction of tyrosine residues in proteins with carbodiimide reagents” Biochim. Biophys. Acta, 160 (1968) 272-274.
Li, Min, et al., “Labeling Monoclonal Antibodies with 90Yttrium—and 111Indium—DOTA Chelates: A Simple and Efficient Method” Bioconjugates Chemistry, Mar./Apr. 1994, vol. 5, No. 2, pp. 101-104.
Griffiths, Gary L., et al., “90Y-DOTA-hLL2: An Agent for Radioimmunotherapy of Non-Hodgkin's Lymphoma” The Journal of Nuclear Medicine, vol. 44, No. 1, Jan. 2003, pp. 77-84.
Meares, Claude, et al., “Conjugation of Antibodies with Bifunctional Chelating Agents: Isothiocyanate and Bromoacetamide Reagents, Methods of Analysis, and Subsequent Addition of Metal Ions” Analytical Biochemistry 142, 68-78 (1984), pp. 68-78.
Kozak, Robert W., et al., “Nature of the Biofunctional Chelating Agent Used for Radioimmunotherapy with Yttrium-90 Monoclonal Antibodies: Critical Factors in Determing in Vivo Survival and Organ Toxicity” Cancer Research 49, 2636-2644, May 15, 1989, pp. 26392644.
Zoghbi, Sami S., et al., “A Modified Procedure for Rapid Labelling of Low Concentrations of Bioactive Proteins with Indium-111” Int. J. Nucl. Med. Biol. vol. 12, No. 3, pp. 159-166, 1985, pp. 159-166.
Esteban, Jose M., et al., “New Method for the Chelation of Indium-111 to Monoclonal Antibodies: Biodistribution and Imaging of Athymic Mice Bearing Human Colon Carcinoma Xenografts” The Journal of Nuclear Medicine, vol. 28, No. 5, May 1987, pp. 861-870.
Harrison, A., et al., “The In Vivo Release of 90Y from Cyclic and Acyclic Ligand—Antibody Conjugates” Nucl. Med. Biol. vol. 18, No. 5, pp. 469-476, 1991.
Hnatowich, D. J., et al., “The Preparation of DTPA-Coupled Antibodies Radiolabeled with Metallic Radionuclides: an Improved Method” Journal of Immunological Methods, 65 (1983) 147-157.
Sharkey, Robert M., et al., “Sselection of a DTPA Chelate Conjugate for Monoclonal antibody Targeting to a Human Colonic tumor in Nude Mide” Int. J. Cancer: 46, 79-85 (1990).
Stimmel, Julie B., et al., “Yttrium-90 Chelation Properties of Tetraazatetraacetic Acid Macrocycles, Diethylenetriaminepentaacetic Acid Analogues, and a Novel Terpyridine Acyclic Clelator” Bioconjugate Chem. 1995, 6, pp. 219-225.
Cummins, Clark H., et al., “A Convenient Sysnthesis of Bifunctional Chelating Agents Based on Diethylenetriaminepentaacetic Acid and Their Coordination Chemistry with Yttrium (III)” Technical Notes Bioconjugate Chem. 1991, 2, 180-186.
Chinn, Paul C., et al., “Preclinical evaluation of 90Y-labeled anti-CD20 monoclonal antibody for treatment of non-Hodgkin's lymphoma” International Journal of Oncology 15: 1017-1025, 1999.
Stimmel, Julie B., et al., “Yttrium-90 Chelation Properties of Tetraazatetraacetic Acid Macrocycles, Diethylenetriaminepentaacetic Acide Analogues, and a Novel Terpyridine Acyclic Chelator” Bioconjugate Chem., 1995, 6, 219-225.
Deshpande, Shrikant V., et al., “Yttrium-90-Labeled Monoclonal Antibody for Therapy: Labeling by a New Macrocyclic Bifunctional Chelating Agent” The Journal of Nuclear Medicine, vol. 31, No. 4, Apr. 1990, pp. 473-479.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for preparing conjugates of proteins and chelating... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for preparing conjugates of proteins and chelating..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing conjugates of proteins and chelating... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3869933

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.