Method for preparing cellular fibronectin

Details Method for preparing cellular fibronectin Method for preparing cellular fibronectin

1997-01-07

1998-05-12

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 701, 435 41, 435404, 435405, C12N 500

Patent

active

057503788

ABSTRACT:
A method for producing cellular fibronectin, said method comprising incubating fibronectin-producing cells in a fibronectin production medium comprising: (a) 1.0-3.5 g/L bicarbonate salt; (b) 1.0-5.0 g/L glucose; (c) 10-30 .mu.g/L dexamethasone; (d) 1-10 g/L hydrolyzed protein; and (e) 5-15 .mu.g/L insulin.

REFERENCES:
patent: 5128321 (1992-07-01), Murray et al.
patent: 5262403 (1993-11-01), Nicolson et al.
patent: 5270300 (1993-12-01), Hunziker
patent: 5354269 (1994-10-01), Goodheart et al.

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