Method for preparing cells for blood analysis

Details Method for preparing cells for blood analysis Method for preparing cells for blood analysis

1986-06-30

1987-04-07

Terapane, John F.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Preparation composition

436 10, 436 63, G01N 3100

Patent

active

046561390

ABSTRACT:
A reagent for blood analysis comprises, as a lysing reagent for leukocyte count, tetradecyltrimethylammonium bromide and/or hexadecyltrimethylammonium chloride, dodecyltrimethylammonium chloride, and citric acid. This reagent is characterized by showing three-peaks fractionation of leukocytes in measuring by means of an automatic blood-analysis instrument. The lysing reagent is used together with a diluent comprising boric acid buffer solution, ethylenediaminetetraacetic acid and (2-pyridylthio-1-oxide) sodium.

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