Method for preparing cell fraction containing hemangioblasts

Details Method for preparing cell fraction containing hemangioblasts Method for preparing cell fraction containing hemangioblasts

2000-11-07

2008-11-25

Qian, Celine X (Department: 1636)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C435S325000

Reexamination Certificate

active

07455962

ABSTRACT:
Mouse PCLP1 was identified by expression cloning with the use of a monoclonal antibody against a surface antigen of a cell line derived from mouse AGM. By fractionating PCLP1-positive/CD45-negative cells and culturing them in vitro, it was clarified that these cells differentiate into endothelial-like cells, angioblast-like cells, and hematopoietic cells. By transferring the PCLP1-positive/CD45-negative cells into a mouse defective in the hematopoietic function, the hematopoietic system was reconstructed over a long period of time. These facts indicate that the PCLP1-positive/CD45-negative cells contain mammalian hemangioblasts capable of expressing the activity as long-term repopulating hematopoietic stem cells (LTR-HSC). The present invention provides a method for preparing a cell fraction containing hemangioblasts, the cell fraction prepared by the method, and use of this cell fraction.

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