Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1987-07-15
1988-04-19
Phillips, Delbert R.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
424101, 514 2, 514834, A61K 3514
Patent
active
047390395
ABSTRACT:
An improved method of recovering antihemophilic factor (AHF) from cryoprecipitated blood plasma is provided. The improved method incorporates the addition of a calcium ion source during the step of cold precipitation of fibrinogen and fibronectin contaminants, as well as the addition of polyethylene glycol (PEG) at one of various stages of the recovery process. PEG may be added during the cold precipitation of fibrinogen and fibronectin, during the precipitation of AHF, or prior to the filtration of AHF solution. The method of the present invention results in a final AHF product having greatly increased solubility, higher specific activity, and lower fibrinogen and fibronectin content.
REFERENCES:
patent: 4069216 (1978-01-01), Shanbrom
patent: 4104266 (1978-08-01), Wickerhauser
patent: 4289691 (1981-09-01), Rock et al.
patent: 4294826 (1931-10-01), Feldman
patent: 4386068 (1983-05-01), Mitra et al.
patent: 4387092 (1983-06-01), Liautaud et al.
patent: 4404131 (1983-09-01), Schwartz et al.
patent: 4406886 (1983-09-01), Bier et al.
patent: 4455301 (1984-06-01), Mitra et al.
patent: 4543210 (1985-09-01), Mitra et al.
Pool and Hershgold, High-potency Antihaemophilic Factor Concentrate etc., Nature, vol. 203, p. 312, Jul. 18, 1964.
Smith et al., A Factor VIII Concentrate of Intermediate Purity etc., Transfusion, vol. 19, No. 3, May-Jun. 1979.
Wickerhauser et al., Development of Large-Scale Fractionation Methods, Vox Sang., 35, pp. 18 to 31 (1978).
Rock et al., Intermediate Purity Factor VIII Production etc., Thrombosis Research, vol. 18, pp. 551-556 (1980).
Marguerie, The Binding of Calcium to Fibrinogen: etc., Biochimica et Biophysica Acta, 494, 172-181 (1977).
Mikaelsson et al., Human Factor VIII: A Calcium-Linked Protein Complex, Blood, vol. 62, No. 5, pp. 1006-1015 (1983).
Co-Sarno Maria E.
Graf Clifford R.
Vasquez Rodolfo A.
Baxter Travenol Laboratories Inc.
Flattery Paul
Hartenberger Robert
Hunter Marjorie
Nutter Nathan M.
LandOfFree
Method for preparing antihemophilic factor (AHF) by cold precipi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for preparing antihemophilic factor (AHF) by cold precipi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing antihemophilic factor (AHF) by cold precipi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2176015