Method for preparing an expanded culture and clonal strains of p

Details Method for preparing an expanded culture and clonal strains of p Method for preparing an expanded culture and clonal strains of p

1995-06-07

1997-07-08

Rollins, John W.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of detaching cells, digesting tissue or establishing...

435383, 435397, C12N 506, C12N 508, C12N 500

Patent

active

056460350

ABSTRACT:
The present invention provides a method for producing an expanded non-transformed cell culture comprising the steps of: (1) preparing partially purified, minced tissue; (2) concentrating the resulting cells and tissue pieces; (3) resuspending the concentrated tissue cells and pieces in a culture medium capable of supporting sustained cell division that is contained in a culture vessel; (4) incubating the cells; and (5) passaging the cells periodically. The present invention further provides clonal strains of cells derived from the above-mentioned cell culture, medium and conditioned medium designed for the culturing of such cells, including pancreatic, thyroid, parathyroid, and parotid cells, and the use of cultured pancreatic cells to form pancreatic pseudotissues composed of matrix-embedded aggregated (pseudoislets) or individual cells, to treat blood sugar disorders in mammals, and to test for cytotoxicity and autoimmune activities with reference to pancreatic endocrine cells.

REFERENCES:
patent: 4673649 (1987-06-01), Boyce et al.
patent: 4940666 (1990-07-01), Boyce et al.
patent: 5143666 (1992-09-01), Ham et al.
patent: 5153132 (1992-10-01), Goodwin et al.
patent: 5155034 (1992-10-01), Wolf et al.
patent: 5308764 (1994-05-01), Goodwin et al.
patent: 5330908 (1994-07-01), Spaulding
patent: 5496722 (1996-03-01), Goodwin et al.
patent: 5523228 (1996-06-01), Ingram et al.
patent: 5545423 (1996-08-01), Soon-Shiong et al.
Freshney, Culture of Animal Cells: A Manual of Basic Technique, pp. 119-143 (1983).
Ambesi-Impiombato et al, Proc. Natl. Acad. Sci USA, 77(6) (Jun. 1980) pp. 3455-3459.
Nielsen, Acta Endocrinol. Suppl (Copenh) vol. 266 (1985) pp. 1-39.
Hulinsky, Transplant Proc. (US), 24(6) (Dec. 1992) p. 2819.
Hu et al., Chinese Medical Journal, 105 (9): 721-725 (1992).
Cooney et al, Diabetologia, V 35, S1, p. A124 (Aug. 1992).
Hellerstrom et al., Diabetes, vol 40 (Suppl. 2), pp. 89-93 (1991).
Tsubouchi et al, Anat. Rec, vol 218, No. 2, pp. 111-115 (1987).
Dunger et al, Horm. Metab. Res., 23: 201-204 (1991).
Brelje et al., "Role of Prolactin Versus Growth Hormone on Islet B-Cell Proliferation in Vitro: Implications for Pregnancy," Endocrinology, 128:45-57 (1991).
Clark et al., "Islet Cell Culture in Defined Serum-Free Medium," Endocrinology, 126:1895-1903 (1990).
Coon et al., "Cell Cultures of Neuroblasts from Rat Olfactory Epithelium that Show Odorant Responses," Proc. Natl. Acad. Sci. U.S.A., 86:1703-1707 (1989).
Feutren et al., "Cyclosporin Increases the Rate and Length of Remissions in Insulin-Dependent Diabetes of Recent Onset," Lancet, 11:119-123 (1986).
Jaworski et al., "Cyclosporin Prophylaxis Induces Long-Term Prevension of Diabetes, and Inhibits Lymphocytic Infiltration in Multiple Target Tissues in the High-Risk BB Rat," Diabetes Res., 3:1-6 (1986).
Mori et al., "Preventive Effects of Cyclosporin on Diabetes in NOD Mice," Diabetologia, 29:244-247 (1986).
R. Paul Robertson, M.D., "Pancreatic and Islet Transplantation for Diabetes --Cures or Curiosities?," New England J. Med., 327:1861-1868 (1992).
Beattie et al., "Functional Impact of Attachment and Purification in the Short Term Culture of Human Pancreatic Islets," Endocrinology, 73:93-98 (1991).
Berry et al., "Bone-Marrow-Derived Chondrogenisis In Vitro," Journal of Cell Science, 101:333-342 (1992).
Braun et al., "Long-Term Treatment With Glucocorticoids Increases Synthesis and Stability of Juntional Acetylcholine Receptors on Innervated Cultured Human Muscle," J. Neurochem., 60:1929-1935 (1993).
Brayden et al., "A Novel Method for Culturing Sweat Gland Epithelia: Comparison of Normal and Cystic Fibrosis Tissues," Br. J. Clin. Pharmac., 29:235-238 (1990).
Carlson et al., "Asynaptic Expression of the Adult Nicotinic Acetylcholine Receptor in Long-Term Cultures of Mammalian Myotubes," Developmental Brain Research, 72:245-252 (1993).
Curcio et al., "Decreased Cultured Endothelial Cell Proliferation in High Glucose Medium is Reversed by Antioxidants: New Insights On the Pathophysiolosical Mechanisms of Diabetic Vascular Complications," In Vitro Cell. Dev. Biol., 28A:787-790 (1992).
de Jong et al., "Serial Culturing of Human Bronchal Epithelial Cells Derived From Biopsies," In Vitro Cell. Dev. Biol., 29A:379-387 (1993).
Dunger et al. "The Effect of Human Amniotic Fluid in Supporting Long-Term Survival of Pancreatic Islets in Tissue Culture," Horm. Metab. Res., 23:201-204 (1991).
Ebert et al., "Establishment and Characterization of Human Renal Cancer and Normal Kdiney Cell Lines," Cancer Research, 50:5531-5536 (1990).
Ethier et al., "Differential Isolation of Normal Luminal Mammary Epithelial Cells and Breast Cancer Cells from Primary and Metastatic Sites Using Selective Media," Cancer Research, 53:627-635 (1993).
Guy et al., "Isolation and Maintenance of the Human Pilosebaceous Duct" 13-cis Retinoic Acid Acts Directly on the Duct In Vitro, British Journal of Dematology, 128:242-248 (1993).
Jaffe et al., "Culture of Human Endothelial Cells Derived From Umbilical Veins," The Journal of Clinical Investigation, 52:2745-2756 (1973).
Jensen et al., "Tissue Culture of Human Epidermal Keratinocytes: A Differentiating Model System for Gene Testing and Somatic Gene Therapy," Journal of Cell Science, 100:255-259 (1991).
Lewis et al., "Use of Microgravity Bioreactors for Development of an In Vitro Rat Salivary Gland Cell Culture Model," Journal of Cellular Biochemistry, 51:265-273 (1993).
Marchetti et al., "In Vitro Function and Xenotransplantation of Long-Term (3 Weeks) Cultured Porcine Islets of Langerhans," Transplantation Proceedings, 24:637 (1992).
Oda et al., "Human Oral Epithelial Cell Culture I. Improved Conditions for Reproducible Culture in Serum-Free Medium," In Vitro Cell. Dev. Biol., 26:589-595 (1990).
Pixley, "Purified Cultures of Keratin-Positive Olfactory Epithelial Cells: Identification of a Subset as Neuronal Supporting (Sustentacular) Cells," Journal of Neuroscience Research, 31:693-707 (1992).
Pronk et al., "A Cobblestone Cell isolated From the Human Omentum: The Mesothelial Cell; Isolation, Identification, and Growth Characteristics," In Vitro Cell. Dev. Biol., 29A:127-134 (1993).
Ray et al., "Proliferation, Differentation, and Long-Term Culture of Primary Hippocampal Neurons," Proc. Natl. Acad. Sci., 90:3602-3606 (1993).
Romagnoli et al., "Treatment of Posterior Hypospadias by the Autologous Graft of Cultured Urethral Epithelium," The New England Journal of Medicine, 323:527-530 (1990).
Rutkowski et al., "Selective Culture of Mitoically Active Human Schwann Cells from Adult Sural Nerves," Annals of Neurology, 31:580-586 (1992).
Solursh, "Formation of Cartilage Tissue In Vitro," Journal of Cellular Biochemistry, 45:258-260 (1991).
Speirs et al., "An Overview of Culture and Isolation Methods Suitable for In Vitro Studies on Pulmonary Neuroendocrine Cells," The Anatomical Record, 236:35-40 (1993).
Vacanti et al., "Tissue-Engineered Growth of Bone And Cartilage," Transplantation Proceedings, 25:1019-1021 (1993).
Volpi et al., "An Efficient Method for Culturing Human Breast Epithelium: Analysis of Results," Tumori, 77:460-464 (1991).
Wilson et al., "Epithelial-Specific Gene Expression During Differentiation of Stratified Primary Human Keratinocyte Cultures," Cell Growth & Differentiation, 3:471-483 (1992).
Wineman et al., "Maintenance of High Levels of Pluripotent Hematopoietic Stem Cells In Vitro: Effect of Stromal Cells and c-kit," Blood 81:365-372 (1993).

Affiliated with

Also associated with

Rating

Method for preparing an expanded culture and clonal strains of p does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for preparing an expanded culture and clonal strains of p, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing an expanded culture and clonal strains of p will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2408386