Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2006-02-28
2006-02-28
Weber, Jon (Department: 1653)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S362000
Reexamination Certificate
active
07005506
ABSTRACT:
For preparing an albumin isolate from a substance containing albumin, the substance is first ground to a flour. The flour is then suspended in an aqueous solution. The albumin is extracted from the flour into the solution by an at least two stage extraction process using at least one protease, at a pH greater than 8 and at a temperature between 30 and 60° C. In the first stage the flour is treated at a lower protease to albumin weight ratio, at a lower pH and at a higher temperature than in the second stage. After the first stage, a first upper flow and a fraction containing the flour are separated, and the albumin is precipitated from said upper flow. The fraction containing the flour is subjected to the second extraction stage. After that, a second upper flow is separated and fed back to the first stage.
REFERENCES:
patent: 4443540 (1984-04-01), Chervan et al.
patent: 4559307 (1985-12-01), Hopkins
patent: 6140469 (2000-10-01), Shen et al.
patent: 1 203 588 (1963-09-01), None
patent: 43 39 743 (1995-08-01), None
patent: 44 29 787 (1996-08-01), None
patent: 0 700 641 (1963-08-01), None
patent: 0 700 641 (1963-08-01), None
patent: 1107229 (1966-08-01), None
patent: 63227171 (1988-09-01), None
patent: WO 95/14394 (1995-06-01), None
Tanaka, T. et al. (2001)-terminal portion acts as an initiator of the inactivation of pepsin at neutral pH. Protein Eng. vol. 14, pp. 669-674.
Zeki Berk, “Technology Of Production Of Edible Flours and Protein Products From Soybeans,” FAO Agricultural Services Bulletin No. 97, Food and Agriculture Organization of the United Nations Rome 1992, http://www.fao.org/docrep/t0532e/to532e07.htm.
Takuji Tanaka and Rickey Y. Yada, “N-terminal portion acts as an initiator of the inactivation of pepsin at neutral pH,” Protein Engineering vol. 14 No. 9 pp. 669-674, 2001.
Liu Samuel Wei
Thomas, Kayden, Horstemeyer and Risley, LLP
Weber Jon
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