Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-03-14
1995-08-08
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 913, 435 915, 536124, C12P 1934, C07H 2102
Patent
active
054398118
ABSTRACT:
A method for preparing aminoacyl- and misaminoacyl-tRNAs utilizing a specific aminoacyl-tRNA hydrolase is provided. According to the present method, aminoacyl-tRNAs consisting of a tRNA and a corresponding amino acid, or misaminoacyl-tRNAs consisting of a tRNA and a non-corresponding amino acid or an amino acid derivative, can be prepared. In a specific embodiment, L-tyrosyl-tRNA.sup.Tyr is prepared using aminoacyl-tRNA hydrolase from Fusarium culmorum or Artemia salina. In another embodiment a misacylated tRNA, L-phenylalanyl-tRNA.sup.Tyr, is prepared using the aminoacyl-tRNA hydrolase.
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Kataoka Chiwa
Miura Kazunobu
Tohyama Junichi
Yamashita Nobuhiko
Naff David M.
Osaka Gas Company Limited
Prats Francisco C.
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