Method for preparing a pesticidally resistant rhizobium and agro

Plant husbandry – Coated or impregnated seed – method or apparatus

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47 58, 195 59, 195 79, 195 96, 195112, C12K 102, C12K 300, A01C 106, A01C 2100

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active

040940977

ABSTRACT:
This invention relates to Rhizobium strains having good infecting and nitrogen-fixing characteristics and which are resistant to fungicides. These strains are produced by cultivating a Rhizobium strain sensitive to a particular fungicide in the presence of an amount of the fungicide, and for a time less than that sufficient to kill the entire Rhizobium population, but sufficient to kill a majority of the population. The remaining Rhizobium population is isolated and recultured in the presence of an increased amount of the fungicide, again an amount and a time less than sufficient to kill the entire Rhizobium population, but sufficient to kill a majority of the population. This procedure is repeated for sufficient passages, with increasing amounts of said fungicide, to provide a Rhizobium strain sufficiently resistant to said fungicide so that the Rhizobium strain multiplies and enters into a nitrogen-fixing symbiosis in the presence of agriculturally effective amounts of the fungicide.

REFERENCES:
patent: 3168796 (1965-02-01), Scott et al.
patent: 3616236 (1971-10-01), Delin
Biswas, et al., "Nodulation of Trifolium alexandrinum by Penicillin Treated Rhizobium trifolli", Chemical Abstracts, vol. 82, No. 5, p. 418, (1975), Abs. No. 30192z.
Namdeo, et al., "Herbicidal Influence on Growth Sensitivity and Mutation Transformation in Rhizobia", Chemical Abstracts, vol. 80, No. 1, p. 235, (1974), Abs. No. 2612b.
Sud, et al., "Growth Promoting Effect of TCA and Dalapon on Rhizobium Strains", Chemical Abstracts, vol. 79, No. 9, p. 284, (1973), Abs. No. 52297c.
Kecskes, et al., "Effect of Some Fungicides on Rhizobium leguminosarum Species", Chemical Abstracts, vol. 79, No. 25, p. 70, (1973), Abs. No. 143388z.
Elek et al., "Effect of Some Seed Treatments Using Micro Elements and Fungicides on Vetch Plants Grown from Seeds Inoculated with Rhizobia", Chemical Abstracts, vol. 79, No. 13, p. 123 (1973) Abs. No. 74560k.
Kecskes, et al., "Compatibility of Fungicide Treatment and Rhizobium Inoculation of Vetch Seed", Chemical Abstract, vol. 79, No. 8, p. 125, (1973), Abs. No. 101547v.

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