Method for preparing a DNA chip and use thereof

Details Method for preparing a DNA chip and use thereof Method for preparing a DNA chip and use thereof

2004-04-19

2010-06-22

Kapushoc, Stephen (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S005000, C435S069100, C435S091100, C435S091400, C435S091520, C536S023100, C536S024200

Reexamination Certificate

active

07741042

ABSTRACT:
The present invention relates to a method for producing a DNA chip, which comprises the steps of: (a) cloning a probe, where a linker is coupled to one or both ends of an oligonucleotide to be integrated on a slide, into a vector; (b) transforming host cells with the vector; (c) culturing the transformed host cells, to recover the probe where the linker is coupled to one or both ends of the oligonucleotides; and (d) integrating the recovered double-helical probes on a slide. Also, the present invention relates to a DNA chip for HPV diagnosis produced by the method, and a method for diagnosing the presence or genotype of HPV using the DNA chip.

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Oh, Taejong et al. , “Development and Clinical Evaulation of a Highly Sensitive DNA Microrray for Detection and Genotyping of Human . . . ”, “Journal of Clinical Microbiology”, Jul. 2004, pp. 3272-3280, vol. 42, No. 7.
Liu, Cui-Hua, et al., Possibility of Using DNA Chip Technology for Diagnosis of Human Papillomavirus , J. Biochem. Mol. Biol., Jul. 2003, pp. 349-353, vol. 36, No. 4.

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