Method for preparing a covalent conjugate of an oligonucleotide

Details Method for preparing a covalent conjugate of an oligonucleotide Method for preparing a covalent conjugate of an oligonucleotide

1993-05-10

1993-12-21

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Stablizing an enzyme by forming a mixture, an adduct or a...

435190, 435191, 435192, 435 6, 435 911, 548546, 548544, C12N 996, C12N 904, C12N 906, C12N 908

Patent

active

052720772

ABSTRACT:
A method is provided for preparing a covalent conjugate of an oligonucleotide and an enzyme, such as peroxidase. This conjugate can be used as a probe in hybridization assays and in polymerase chain reaction procedures. The method generally comprises the steps of: reacting an enzyme having a reactive amino group with a mercapto-substituted organic compound to form a blocked intermediate, removing the blocking group to form an enzyme reagent, and reacting the enzyme reagent with a functionalized oligonucleotide reagent.

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