Method for preparing a cell free homogenate of Chrysanthemum cin

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435155, 435188, 435195, 435196, 435178, C12P 762, C12P 702, C12N 914

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045254559

ABSTRACT:
A method is disclosed for preparing a cell free homogenate from Chrysanthemum species and Tagetes species of the family Compositae which contains the bioactive enzyme(s) and cofactor(s) of the pyrethrum pathway. A method is also disclosed for preparing a cell free matrix containing these bioactive enzyme(s) and cofactor(s). The homogenate and matrix will convert mevalonic acid, labeled mevalonic acid, isopentenyl pyrophosphate or labeled isopentenyl pyrophosphate into pyrethrins, labeled pyrethrins, chrysanthemyl alcohol or labeled chrysanthemyl alcohol.

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