Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Reexamination Certificate
2007-09-11
2007-09-11
Achutamurthy, Ponnathapu (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
C435S183000, C435S252300, C435S320100, C536S023200
Reexamination Certificate
active
10043639
ABSTRACT:
The invention concerns a method for preparing 1,3-propanediol from a carbon-containing substance, said method comprising a step which consists in culturing a recombinant micro-organism not producing coenzyme B12 in the absence of coenzyme B12 or one of its precursors. The invention also concerns a nucleic acid coding for a glycerol dehydratase whereof the catalytic activity is independent of the presence of coenzyme B12 or one of its precursors and a nucleic acid coding for a 1,3-propanol dehydrogenase intervening in the synthesis of 1,3-propanediol. The invention further concerns recombinant vectors and host cells comprising said nucleic acids and the polypeptides coded by the latter.
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patent: 5686276 (1997-11-01), Laffend et al.
patent: 5821092 (1998-10-01), Nagarajan et al.
patent: WO 98/21339 (1998-05-01), None
Luers et al., “Glycerol conversion to 1.3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1.3-propanediol dehydrogenase,”FEMS Microbiology Letters. 154:337-345, 1997.
Macis et al., “Properties and sequences of the coenzyme B12-dependent glycerol dehydratase ofClostridium pasteurianum,” FEMS Microbiology Letters. 164:21-28, 1998.
Reimann et al., “1.3-propanediol formation with product-tolerant mutants ofCostridium butyricumDSM 5431 in continuous culture: productivity, carbon and electron flow.”J. Applied Microbiology. 84:1125-1130. 1998.
Skraly et al., “Construction and characterization of a 1.3-propanediol operon.”Applied and Environmental Microbiology. 64(1):98-105. 1998.
Weidner and Sawers. “Molecular characterization of the genes encoding pyruvate formate-lyase and its activating enzyme ofClostridium pasteurianum.”J. of Bacteriology. 178(8):2440-2444. 1996.
Croux Christian
Sarcabal Patricia
Soucaille Philippe
Achutamurthy Ponnathapu
Fronda Christian L.
Fulbright & Jaworski L.L.P.
Institut National de la Recherche
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