Method for preparing...

Details Method for preparing... Method for preparing...

2011-04-26

2011-04-26

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S052000, C435S171000

Reexamination Certificate

active

07932057

ABSTRACT:
The present invention provides a method for preparing 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol (Ginsenoside M1) by using Sanqis leaves and stems. The method of the invention includes the steps of: (a) provides powder of Sanqi leaves and stems; (b) provides a fungus for fermenting the Sanqi leaves and stems, wherein the fermentation temperature is ranged from 20-50° C., the fermentation humidity is ranged from 70-100%, the pH value is ranged from 4.0-6.0, and the fermentation period is ranged from 5-15 days; (c) extracts and collects the fermentation products; and (d) isolates 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol from the fermentation products.

REFERENCES:
patent: I280982 (2007-05-01), None
Zhang “Assay of total glycoside in San Qi (Panax notoginseng) and its preparation with macroreticular resins” Zhongcaoyao (1981) 12(11): 503-505 (abstract only).
San Qi from Chinese Medicine Tools downloaded from tcm.health-infor.org/Herbology.Materia.Medica/sanqi-properties.htm on Aug. 25, 2009.
Du et al. “Synthesis of oligosaccharide derivatives related to those from sanqi, a Chinese herbal medicine from Panax notoginseng” Carb. Res 337 (2002): 485-491.
Printout from the Registry file showing the strucutre of Panaxzadiol. downloaded from STN on Aug. 26, 2009.
English translation of TW I280982 downloaded from Google translations on Aug. 6, 2010, 7 pages.
STN CAPLUS abstract for TW 280982 down loaded from CAPLUS Aug. 12, 2010, 1 page.

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