Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Peptides containing saccharide radicals – e.g. – bleomycins – etc.
Patent
1989-07-18
1991-04-02
Lee, Lester L.
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
Peptides containing saccharide radicals, e.g., bleomycins, etc.
C07K 900
Patent
active
050048180
ABSTRACT:
In a method for preparing nephritogenoside from human placenta, rat kidney or other organs, tissues containing many microvessels are excised from the organ and treated with a nonionic surface active agent, especially, Triton X 100. Thus obtained precipitates are digested with trypsin and subjected to a chromatography treatment. Introduction of the nonionic surface active agent treatment enables simplification of the digestion step as well as a drastic improvement in yield.
REFERENCES:
patent: 4382887 (1983-10-01), Shibata
S. Shibata, "Basement Membranes", Proceedings of the International Symposium on Basement Membranes, held in Mashimo (Japan) on Jun. 24-26, 1985, pp. 25-37, published by Elsevier Science Publishers, Amsterdam, 1985.
Connective Tissue Research, 1987, vol. 16, pp. 153-162.
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