Method for preparation of human serum albumin-sensitized sheep e

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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424533, 436521, 436820, A61K 3518

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053169360

ABSTRACT:
A method for preparation in a large quantity of human serum albumin-sensitized sheep erythrocytes is described. The sensitized cells are useful for detecting Hepatitis B virus pre-S.sub.2 antigen. The method uses human serum albumin sensitized glutaraldehyde-fixed, sheep erythrocytes in the presence of chromium chloride.

REFERENCES:
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Basmadzhyan et al., Zh Eksp Klin Med 19(3):56-58 (1979) Abstract BA71:31277.
Derechiskaya et al., Lab Delo 0(12):25-29 (1989) Abstract BA90:40107.
A. Machida, et al., "A Hepatitis B Surface Antigen Polypeptide (P32) With the Receptor for Polymerized Human as Well as Chimpanzee Albumins", Gastroenterology, vol. 85, No. 2, 1983.
Y. Itoh, et al., "A Synthetic Peptide Vaccine Involving the Product of the Pre-S(s) Region of Hepatitis B Virus DNA: Protective Efficacy in Chimpanzees", Proc. Natl. Acad. Sci. U.S.A., vol. 83, 1986.
P. Tiollais, et al., "Biology of Hepatitis B Virus", Science, vol. 213, 1981.
M. Imai, et al., "A Receptor for Polymerized Human and Chimpanzee Albumins on Hepatitis B Virus Particles Co-occuring with HBeAg", Gastroenterology, vol. 76, No. 2, 1979.
H. Okamoto, et al., "Hemagglutination Assay of Polypeptide Coded by the Pre-S Region of Hepatitis B Virus DNA with Monoclonal Antibody: Correlation of Pre-S Polypeptide with the Receptor for Polymerized Human Serum Albumin in Serums Containing Hepatitis B Antigens", The Journal of Immunology, vol. 134, No. 2, 1985.

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