Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Plant proteins – e.g. – derived from legumes – algae or...
Patent
1984-02-29
1986-05-13
Wiseman, Thomas G.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Plant proteins, e.g., derived from legumes, algae or...
260112R, 536128, 4241951, 530379, 435816, C12N 988, A61K 3578, C08G 802
Patent
active
045886911
ABSTRACT:
An improved method for the purification of ribulose, 1,5-bisphosphate carboxylase (RuBisCO) comprises comminuting and homogenizing a plant material, such as leaves, in an aqueous solution. After fractionation to release the RuBisCO, sufficient polyethylene glycol (PEG) is added to cause crystallization of the RuBisCO. It has been found that treatment of the resulting PEG supernatant first by acidification to remove other proteins, and then by addition of a strong base to remove phosphorylated sugars, allows the recycling of the PEG in the process. Moreover, it is found that the phosphorylated sugars are a valuable by-product, suitable for example as a carbon source for the culture of microorganisms.
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The Merck Index, published by Merck & Co. Inc., 1976.
Sarjit Johal and Don P. Bourque (1979) Science 204:75-77 "Crystalline Ribulose 1,5-Bisphosphate Carboxylase . . . from Spinach".
Janet Paulsen and M. D. Lane (1966) Biochem. 5:2350-2357 "Spinach Ribulose Diphosphate Carboxylase . . . of the Enzyme".
Teskin Robin Lyn
Wiseman Thomas G.
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