Method for preparation of androst-4-ene-3,17-dione and...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a...

Reexamination Certificate

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C435S253100, C435S244000

Reexamination Certificate

active

10477448

ABSTRACT:
The present invention relates to a method for preparation of androst-4-ene-3,17-dione (AD) and androsta-1,4-diene-3,17-dione (ADD). More particularly, the present invention relates to a method for preparing AD/ADD, comprising the steps of: (a) heating sterols and emulsifier respectively, to dissolve completely and mixing to prepare a mixture; (b) placing the mixture in a water bath at 70˜90° C. and stirring to obtain emulsified sterols; and (c) adding the emulsified sterols to culture media of microorganism as a component. And the present invention relates to a method for preparing AD/ADD, comprising the addition of cyclodextrin-sterol complexes extracted from milk to culture media of microorganism as a component. The emulsified sterols and cyclodextrin-sterol complexes adopted in the present invention can be easily prepared and be effectively used for obtaining AD/ADD at a high yield.

REFERENCES:
patent: 4097334 (1978-06-01), Weber et al.
patent: 4212940 (1980-07-01), Weber et al.
patent: 4397946 (1983-08-01), Imada et al.
patent: 4528271 (1985-07-01), Udvardy Nagy nee Cserey Pechany et al.
patent: 5418145 (1995-05-01), Weber et al.
patent: 57-08794 (1982-01-01), None
patent: WO 87-03620 (1987-06-01), None

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