Method for preparation of a recombinant DNA phage

Details Method for preparation of a recombinant DNA phage Method for preparation of a recombinant DNA phage

1979-10-22

1982-09-07

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435235, 435317, L12N 1500

Patent

active

043484786

ABSTRACT:
A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.

REFERENCES:
Murray et al., Nature, vol. 251, pp. 476-481, Oct. 1974.
Scott, Molecular Cloning of Recombinant DNA, pp. 133-153 (1977).
Levin, Gene Expression-3, pp. 269, 270, 879, 886 and 901, (1977).
Thomas et al., Proc. Nat. Acad. Sci. USA, vol. 71, No. 11, pp. 4579-4583 (1974).

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