Method for predicting responsiveness to TNFα blocking...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C536S023500, C536S024310

Reexamination Certificate

active

07985549

ABSTRACT:
The invention provides an in vitro method for predicting whether a patient would be responsive to a treatment with a TNFα blocking agent, which method comprises determining the expression level of eight genes in a biological sample of said patient, wherein said genes are EPS15, HLA-DPB1, AKAP9, RASGRP3, MTCBP-1, PTNP12, MRPL22 and RPS28. The invention further provides a DNA chip for performing such method.

REFERENCES:
patent: 2005/0196799 (2005-09-01), Wigler et al.
R. Maini et al., “Infliximab (chimeric anti-tumour necrosis factor alpha monoclonal antibody) versus placebo in rheumatoid arthritis patients receiving concomitant methotrexate: a randomized phase III trial”, The Lancet Limited, London, GB, vol. 354, No. 9194, Dec. 4, 1999, pp. 1932-1939.
T. Lequerre et al., “Gene expression profiling in PBMCs as a tool for prediction of infliximab responsiveness in rheumatoid arthritis”, Arthritis & Rheumatism, vol. 50, 2004, p. S398.
T. Lequerre et al., “Prediction of infliximab responsiveness in rheumatoid arthritis from gene profiling in PBMCs, but not with autoantibodies (autoab), metalloproteinases and bone markers parameters in sera of patients”, Arthritis & Rheumatism, vol. 52, No. 9S, Sep. 2005, pp. S569-S570.

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