Method for plant protection against insects or nematodes by...

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

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Details

C800S279000, C800S265000, C435S320100

Reexamination Certificate

active

06861578

ABSTRACT:
A method of protecting a plant or part of a plant from insect or nematode infestation is disclosed herein. The method includes the steps of: inserting into the genome of cells or tissue from a plant a sequence coding for equistatin operably linked to a promoter sequence active in the plant to cause expression of the protein at levels which provide an insect or nematode controlling amount of the protein; and regenerating resistant whole plants from the cells or tissue. Also disclosed is a transgenic plant or its sexual progeny which is resistant to attack by one or more insects or nematodes having digestive cysteine proteases. Also disclosed is an expression vehicle that includes a promotor effective to promote expression of a downstream coding sequence in plant cells, the expression vehicle being effective to express in plant cells insect controlling amounts of equistatin.

REFERENCES:
patent: PCTEP95/00881 (1995-03-01), None
patent: 9221753 (1992-12-01), None
Lazar et al., Transforming Growth Factor x: Mutation of Aspartic Acid 47 and Leucine 48 Result in Different Biological Activities, Mar. 1988, Molecular and Cellular Biology, vol. 8, No. 3, pp. 1247-1252.*
Hill et al., Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase fromEschetichia coli,1998, Biochemical and Biophysical Research Comm., vol. 244, pp. 573-577.*
Lenarcic, Brigita; Ritonja, Anka; Strukelj, Borut; Turk, Boris; and Turk, Vito. “Equistatin, a New Inhibitor of Cysteine Proteinases from Actinia equina, Is Structurally Related to Thyroglobulin Type-1 Domain.” The Journal of Biological Chemistry, vol. 272, No. 21, pp. 13899-13903 and Addition or Correction Sheet (May 23, 1997).
Yamashita, Michiaki and Konagaya, Shiro. “A Novel Cysteine Protease Inhibitor of the Egg of Chum Salmon, Containing a Cysteinerich Thyroglobulin-like Motif.” The Journal of Biological Chemistry, vol. 271, No. 3, pp. 1282-1284 (Jan. 19, 1996).

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