Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2005-03-01
2005-03-01
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S279000, C800S265000, C435S320100
Reexamination Certificate
active
06861578
ABSTRACT:
A method of protecting a plant or part of a plant from insect or nematode infestation is disclosed herein. The method includes the steps of: inserting into the genome of cells or tissue from a plant a sequence coding for equistatin operably linked to a promoter sequence active in the plant to cause expression of the protein at levels which provide an insect or nematode controlling amount of the protein; and regenerating resistant whole plants from the cells or tissue. Also disclosed is a transgenic plant or its sexual progeny which is resistant to attack by one or more insects or nematodes having digestive cysteine proteases. Also disclosed is an expression vehicle that includes a promotor effective to promote expression of a downstream coding sequence in plant cells, the expression vehicle being effective to express in plant cells insect controlling amounts of equistatin.
REFERENCES:
patent: PCTEP95/00881 (1995-03-01), None
patent: 9221753 (1992-12-01), None
Lazar et al., Transforming Growth Factor x: Mutation of Aspartic Acid 47 and Leucine 48 Result in Different Biological Activities, Mar. 1988, Molecular and Cellular Biology, vol. 8, No. 3, pp. 1247-1252.*
Hill et al., Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase fromEschetichia coli,1998, Biochemical and Biophysical Research Comm., vol. 244, pp. 573-577.*
Lenarcic, Brigita; Ritonja, Anka; Strukelj, Borut; Turk, Boris; and Turk, Vito. “Equistatin, a New Inhibitor of Cysteine Proteinases from Actinia equina, Is Structurally Related to Thyroglobulin Type-1 Domain.” The Journal of Biological Chemistry, vol. 272, No. 21, pp. 13899-13903 and Addition or Correction Sheet (May 23, 1997).
Yamashita, Michiaki and Konagaya, Shiro. “A Novel Cysteine Protease Inhibitor of the Egg of Chum Salmon, Containing a Cysteinerich Thyroglobulin-like Motif.” The Journal of Biological Chemistry, vol. 271, No. 3, pp. 1282-1284 (Jan. 19, 1996).
Bosch Hendrik J.
Gruden Kristina
Jongsma Maarten Anthonie
Lenarcic Brigita
Stiekema Willem Johannes
Kubelik Anne
Plant Research International B.V.
Thomas Kayden Horstemeyer & Risley
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