Method for photometric determination of the subunit B of creatin

Details Method for photometric determination of the subunit B of creatin Method for photometric determination of the subunit B of creatin

1979-06-21

1980-11-25

Therkorn, Ernest G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 17, 435184, 435810, C12Q 166

Patent

active

042359619

ABSTRACT:
A method for the photometric determination of the subunit B of creatine kinase (CK-B) in buffered water solution through reaction with creatine phosphate, ADP and the system luciferase/D-luciferin in the presence of a specific antibody inhibiting the subunit M of creatine kinase, comprises making the determination in the presence of a D-luciferin analog, such as L-luciferin, having a structure similar to D-luciferin and being a competitive inhibitor of the firefly luciferase reaction.
Also disclosed is a reagent for carrying out the above method comprising creatine-phosphate, ADP, luciferase, D-luciferin, L-luciferin, CK-M inhibiting antibodies, diadenosinpentaphosphate, SH-reagent, magnesium salt and buffer as well as possibly stabilizers and/or complex forming reagent.

REFERENCES:
patent: 3929580 (1975-12-01), Forgione
patent: 3933592 (1976-01-01), Clendenning
patent: 4001088 (1977-01-01), Antonik
patent: 4067775 (1978-01-01), Wuezburg
patent: 4080265 (1978-03-01), Antonik
"Determination of Creatine Kinase Isoenzyme MB Activity in Serum Using Immunological Inhibition of Creative Kinase", Subunt Activity, Neumier Climica Chimica Acta 73 (1976) 445-451.
"Immobilized Firefly Luciferase", Lee, Analytical BioChemistry 80, (1977), pp. 490-501.

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