Method for performing polynucleotide separations using liquid ch

Details Method for performing polynucleotide separations using liquid ch Method for performing polynucleotide separations using liquid ch

1998-05-18

2000-01-25

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 210659, 435 6, 536 231, 536 254, 935 18, 935 77, B01D 1508

Patent

active

060174579

ABSTRACT:
Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

REFERENCES:
patent: 4563510 (1986-01-01), Ugelstad
patent: 4683202 (1987-07-01), Mullis
patent: 4855225 (1989-08-01), Fung et al.
patent: 5338448 (1994-08-01), Gjerde
patent: 5585236 (1996-12-01), Bonn et al.
patent: 5616701 (1997-04-01), Woodard et al.
patent: 5705628 (1998-01-01), Hawkins
Rouhi et al, C & EN Washington, (Mar. 16, 1998) pp. 46 and 48.
Transgenomic, Inc. Product Bulletin, pp. 1-8 undated.
J. W. Goodwin et al., Colloid & Polymer Sci., 252:464-471 (1974).
M. Herold et al., BioChromatography, 10:656-662 (1991).
J. Hirabayashi et al., Anal. Biochem., 178:336-341 (1989).
J. Hirabayashi et al., Biochemistry, 29:9515-9521 (1990).
Huber et al., Chomatographia, 37:653-658 (1993).
Y. Kato et al., J. Chromatogr., 478:264-268 (1989).
Y. Ohimya et al., Anal. Biochem., 189:126-130 (1990).
R. K. Saiki et al., Science, 230:1350-1354 (1985).
J. Ugelstad et al., Adv. Colloid Interface Sci., 13:101-140 (1980).
All-Chrom Newsletter, 25:1-6 (Jun. 1986).
Eriksson,s. et al. Separation of DNA Restriction Franments by Ion-Pair Chromatography. Journal of Chromatography. Jan. 1986, vol. 359, pp. 265-274.
Huber et al. High-Resolution Liquid Chromatography of Oligonucleotides on nonporous Alkylated Styrene-Divinylbenzene Copolymers. Analytical Biochemistry. 1993 vol. 212. pp. 351-358.

Affiliated with

Also associated with

Rating

Method for performing polynucleotide separations using liquid ch does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for performing polynucleotide separations using liquid ch, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for performing polynucleotide separations using liquid ch will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2313483