Chemistry: analytical and immunological testing – Including use of radioactive properties
Patent
1991-05-30
1993-03-30
Housel, James C.
Chemistry: analytical and immunological testing
Including use of radioactive properties
250328, 250364, G01N 2300
Patent
active
051983648
DESCRIPTION:
BRIEF SUMMARY
The object of the invention is a method for performing liquid scintillation counting by the liquid scintillation counter that measures radioactive samples on a measurement support, such as a filter plate or alike, which has the sample associated therein, according to which method the detection material, such as scintillator, is added to the measurement support before the measurement.
The invention relates to liquid scintillation counting directly on a filter plate. A liquid scintillation counter of this kind is manufactured by e.g. the applicant Wallac Oy with the trade name 1205 Betaplate. With this instrument samples labeled with radioactive markers are measured, said samples being located on a filter plate or on other corresponding plate. The size of the filter plate used by the applicant is about 10 cm.times.25 cm. The samples are situated on the plate in 16 rows so that each row contains 6 samples. The total number of samples is 96. Each sample covers an area with the diameter of 9 mm.
According to a known method the filter plate having the samples to be measured is put into a flat plastic bag and about 10 ml of liquid scintillant is added. The bag is sealed e.g. with a heat sealer and inserted into a measureing cassette of 1205 Betaplate. The liquid scintillant converts part of the radioactive energy emitted from the samples into light that is measured by the photomultiplier tubes of the liquid scintillation counter.
A drawback with this known method has been that in some cases the samples on the filter plate have been noticed to gradually dissolve into the liquid scintillant. If the samples then get intermixed, serious errors are introduced in the measurement.
The object of the present invention is to produce a new method and a new detection material for liquid scintillation counting. The method according to the invention is characterized by adding detection material, such as scintillator, in melted form into the measurement support and performing the measurement after the detection material has solidified.
The detection material according to the invention is characterized in that said detection material is easily melting material that solidifies before the measurement. A plateshaped solid scintillating plate to be melted into the filter plate removes the problem mentioned above, because the samples do not dissolve in the solidified scintillating plate.
The other characteristics of the invention are presented in the patent claims below.
The invention is explained with enclosed drawings wherein
FIG. 1 presents detection material plate and filter plate on which the samples are situated.
FIG. 2 presents the plates of FIG. 1 placed on each other.
FIG. 3 presents the filter plate of FIG. 2 having the detection material plate melted therein.
The detection material according to the invention is used in the following way:
The filter plate 1, on which the samples to be determined reside, is put into a flat plastic bag, into which the mentioned solid detection material, such as a scintillating plate, is added, said scintillating plate being of about same size as the filter plate. The thickness of the scintillating plate is e.g. about 1-2 mm. After this the solid scintillating plate 3 is melted into the porous filter plate with e.g. a hot metal plate. Upon melting the liquid formed by the scintillating plate penetrates so close to the radioactive sample molecules that the radiation and the scintillator material of the scintillating plate can interact. After melting the system is cooled to room temperature, whereafter the material of the scintillating plate is solid again and the samples can not dissolve into it. As a result of interaction process part of the radiation energy emitted by molecules is converted to light that is measured with the mentioned liquid scintillation counter.
According to an advantageous embodiment of the scintillating plate, it consists of at least two components, of which at least one acts as a so called carrier and the other components, the number of which is at least one, act as actu
REFERENCES:
patent: 4488046 (1984-12-01), Bernstein
patent: 4764677 (1988-08-01), Spurney
patent: 4972084 (1990-11-01), Potter et al.
Oikari Timo
Yrjonen Tapio
Housel James C.
Wallac Oy
Wallenhorst Maureen M.
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