Method for performing heterogeneous immunoassay

Details Method for performing heterogeneous immunoassay Method for performing heterogeneous immunoassay

1987-11-03

1991-04-23

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435174, 435177, 435180, 435 794, 436501, 436518, 436527, 436538, 436529, 436531, 436807, 436810, 436824, 436825, G01N 3353, G01N 33543, G01N 33545, C12N 1100

Patent

active

050099981

ABSTRACT:
The method described coats the interior of a tube-like support with the binding partner of the analyte. Analyte and anti-analyte-label conjugate are added. When the tube-like support is rotated end-over-end, the thin layer of analyte and conjugate readily react with the binding partners to form an immobilized label complex. When rotation stops, the reaction quenches to provide precise timing. The label complex is released and measured.

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C. J. Van Oss et al., Sep. Purif. Methods, vol. 7, 245, (1978).
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