Method for optically measuring chemical analytes

Chemistry: analytical and immunological testing – Blood gas

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436 74, 436163, 436172, 128633, G01N 2180

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active

056248474

ABSTRACT:
A system and method in which a photoluminescent ligand is added to a sample to be analyzed in the form of a photoluminescent probe having intrinsic analyte-induced lifetime changes. The method preferably employs phase-modulation fluorometry to measure the lifetime changes. Specific probes are disclosed for measuring various analytes, particularly ionic solutes, including H.sup.+, Ca.sup.2+ and K.sup.+.

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patent: 5308581 (1994-05-01), Lippitsch et al.
Miyoshi et al., "A New Method of Determining Intracellular Free Ca2+ Concentration Using Quin2-Fluorescence" Photochemistry and Photobiology, vol. 53, pp. 415-418 (1991).
"Bioprobes", Molecular Probes, Inc., pp. 1-16 (1988).
Gehrich, J.L. et al., IEEE Transactions on Biomedical Engineering, vol. BME-33, No. 2 (1986) pp. 117-132.
Lippitsch, M.E. et al., Analytica Chimica Acta, vol. 205 (1988) pp. 1-6.
Lakowicz, J. et al., Applications of Fluorescence in the Biomedical Sciences (1986) pp. 29-67.

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